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36F-18C [36F]
36F-18C [36F]
規格:
價格:
編號:B163718
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
36F-18C [36F]
商品貨號
B163718
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for sheep CD2 and precipitates a 50000 to 55000 dalton molecule from lysates of sheep lymphocytes.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against sheep (ovine) CD2
Cellular Products
immunoglobulin; monoclonal antibody; against sheep (ovine) CD2
Comments
Animals were immunized with phytohemagglutinin-stimulated sheep T cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for sheep CD2 and precipitates a 50000 to 55000 dalton molecule from lysates of sheep lymphocytes.
It does not inhibit cytotoxic T lymphocyte mediated killing or mixed lymphocyte reactivity.
Complete Growth Medium
CULTURE MEDIUM: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum,
10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgG2a
Name of Depositor
CR Mackay
Deposited As
mouse (B cell); mouse (myeloma)
References

Mackay CR, et al. Unusual expression of CD2 in sheep: implications for T cell interactions. Eur. J. Immunol. 18: 1681-1688, 1988. PubMed: 2462499

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