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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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35.1
35.1
規格:
價格:
編號:B163715
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
35.1
商品貨號
B163715
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with the human T cell surface protein Tp50 (CD2).
Derivation
Spleen cells were fused with NSI/1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD2
Cellular Products
immunoglobulin; monoclonal antibody; against human CD2
Comments
Animals were immunized with human T lymphocytes that had been activated with phorbol myristic acid (PMA).
Spleen cells were fused with NSI/1 myeloma cells.
The antibody reacts with the human T cell surface protein Tp50 (CD2).
The antibody inhibits T cell proliferative responses and cytotoxicity, but does not inhibit antibody mediated cellular cytotoxicity.
Complete Growth Medium
RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and 2 mM L-glutamine, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.
Isotype
IgG2a
Name of Depositor
PJ Martin
Deposited As
mouse (B cell); mouse (myeloma)
References

Martin PJ, et al. Identification and functional characterization of two distinct epitopes on the human T cell surface protein Tp50. J. Immunol. 131: 180-185, 1983. PubMed: 6190907

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