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32D Clone 3
32D Clone 3
規(guī)格:
價(jià)格:
編號(hào):B163704
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
32D Clone 3
商品貨號(hào)
B163704
Organism
Mus musculus, mouse
Tissue
bone marrow
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
mixed, adherent and suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
This cell line is a suitable transfection host.
Storage Conditions
liquid nitrogen vapor temperature
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Comments
This line is IL-3 dependent.
The line was originally contaminated with mycoplasma, and was cured by treatment with BM-cycline.
The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.
Complete Growth Medium
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with: 10% heat-inactivated fetal bovine serum 10% mouse Interleukin-3 culture supplement (available from Becton Dickinson Cat. No. 354040, formerly Collaborative Research Cat. No. 40040)
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 105 viable cells/mL. Maintain cell density between 2 X 105 and 1 X 106 viable cells/mL. Scrape off the attached cells and transfer along with the floating cells into new flasks.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
Name of Depositor
Amgen, Inc.
Deposited As
Mus musculus
U.S. Patent Number
References

Ahmed N, Berridge MV. Regulation of glucose transport by interleukin-3 in growth factor-dependent and oncogene-transformed bone marrow-derived cell lines. Leuk. Res. 21: 609-618, 1997. PubMed: 9301681

Ahmed N, Berridge MV. Distinct regulation of glucose transport by interleukin-3 and oncogenes in a murine bone marrow-derived cell line. Biochem. Pharmacol. 57: 387-396, 1999. PubMed: 9933027

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