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3197-3 (3T3-MSV)
3197-3 (3T3-MSV)
規(guī)格:
價(jià)格:
編號(hào):B163700
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
3197-3 (3T3-MSV)
商品貨號(hào)
B163700
Organism
Mus musculus, mouse
Tissue
embryo
培養(yǎng)基
Cell Type
fibroblast
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
sarcoma
Age
embryo
Applications
The cells were derived from ATCC CCL-92 by transformation with Moloney murine sarcoma virus.
They do not produce virus particles.
Tested and found negative for ectromelia virus (mousepox).
Derivation
The cells were derived from ATCC CCL-92 by transformation with Moloney murine sarcoma virus.
Genes Expressed
The cells release a growth factor in serum free medium.
Cellular Products
The cells release a growth factor in serum free medium.
Comments
The cells were derived from ATCC CCL-92 by transformation with Moloney murine sarcoma virus.
They do not produce virus particles.
The cells have a tendency to slough off the flask surface.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Dulbecco's modified Eagle's medium, 95%; bovine calf serum, 5%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:8 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, add fresh 0.25% trypsin solution for 5 minutes, add fresh medium, aspirate and dispense into new flasks. Subculture every 3 to 6 days.
Name of Depositor
JE DeLarco
Deposited As
Mus musculus
References

de Larco JE, Todaro GJ. Growth factors from murine sarcoma virus-transformed cells. Proc. Natl. Acad. Sci. USA 75: 4001-4005, 1978. PubMed: 211512

Bassin RH, et al. Isolation of murine sarcoma virus-transformed mouse cells which are negative for leukemia virus from agar suspension cultures. Int. J. Cancer 6: 95-107, 1970. PubMed: 4319233

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