Comments
Animals were immunized with a synthetic peptide with the sequence LMRACWQWNPSDRPSF corresponding to residues 471-486 of the abl oncogene (formerly 590 to 605 of the v-abl oncogene).
Spleen cells were fused with Sp2/0 myeloma cells.
In ELISA testing, the antibody recognizes the fes, mos, erb B, src, fgr, yes, mil, raf, fms, and insulin receptor sequences in addition to the abl sequence. In immunoblot testing, a protein of approximately 80 kd is recognized in MOLT-4 cells.
The IgG1 reactivity of the antibody with the abl peptide (471 to 486) was confirmed by ELISA. IgG1 was strongly positive while IgG2b was negative.
