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當(dāng)前位置: 首頁 > ATCC代理 > 30-5-7S B163694
30-5-7S
30-5-7S
規(guī)格:
價格:
編號:B163694
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
30-5-7S
商品貨號
B163694
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The reactivity corresponds to the H2.65 serological specificity.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against L d, D q, L q an d L b; cytotoxic
Cellular Products
immunoglobulin; monoclonal antibody; against L d, D q, L q an d L b; cytotoxic
Comments
Animals were immunized with spleen cells and tail skin from BALB/c mice.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The reactivity corresponds to the H2.65 serological specificity.
The antibody is cytotoxic.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
    • Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Isotype
    IgG2a; kappa light chain
    Name of Depositor
    DH Sachs, K Ozato
    Deposited As
    mouse (B cell); mouse (myeloma)
    References

    Ozato K, et al. Monoclonal antibodies to mouse MHC antigens. II. Antibodies to the H-2 Ld antigen, the products of a third polymorphic locus of the mouse major histocompatibility complex. J. Immunol. 125: 2473-2477, 1980. PubMed: 7191868

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