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2D-7
2D-7
規格:
價格:
編號:B163679
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
2D-7
商品貨號
B163679
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody reacts with a major epitope on human CENP-B.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Clinical Data
The binding of the 2D-7 antibody is at least partially blocked by sera from patients with anticentromere antibodies.
Genes Expressed
immunoglobulin; monoclonal antibody; against human centromere protein B (CENP-B)
Cellular Products
immunoglobulin; monoclonal antibody; against human centromere protein B (CENP-B)
Comments
Animals were immunized with human CENP-B peptide.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with a major epitope on human CENP-B.
The binding of the 2D-7 antibody is at least partially blocked by sera from patients with anticentromere antibodies.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
Johns Hopkins University
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Earnshaw W, et al. Cloned human centromere autoantigen. US Patent 5,196,307 dated Mar 23 1993

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