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2D4
2D4
規格:
價格:
編號:B163678
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
2D4
商品貨號
B163678
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with a distinct steric portion of the carbohydrate moiety of asialo GM2, specifically the C6 hydroxyl bearing group of the terminal N-acetylgalactosamine residue.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against asialo GM2
Cellular Products
immunoglobulin; monoclonal antibody; against asialo GM2
Comments
Animals were immunized with asialo GM2 non-covalently adsorbed to naked Salmonella minnesota.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with a distinct steric portion of the carbohydrate moiety of asialo GM2, specifically the C6 hydroxyl bearing group of the terminal N-acetylgalactosamine residue.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
RPMI 1640 medium, 85%; fetal bovine serum, 15%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype
IgM
Name of Depositor
WW Young
Deposited As
mouse (B cell); mouse (myeloma)
References

Young WW Jr., et al. Production of monoclonal antibodies specific for two distinct steric portions of the glycolipid ganglio-N-triosylceramide (Asialo GM2). J. Exp. Med. 150: 1008-1019, 1979. PubMed: 512581

Young WW J, Hakomori SI. Therapy of mouse lymphoma with monoclonal antibodies to glycolipid: selection of low antigenic variants in vivo. Science 211: 487-489, 1981. PubMed: 7455688

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