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2D12
2D12
規格:
價格:
編號:B163677
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
2D12
商品貨號
B163677
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions
liquid nitrogen vapor phase
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Antigen Expression
H-2d
Genes Expressed
immunoglobulin; monoclonal antibody; against yellow fever virus (vaccine strains and Asibi strain),H-2d
Cellular Products
immunoglobulin; monoclonal antibody; against yellow fever virus (vaccine strains and Asibi strain)
Comments
Mice were immunized with the 17D strain of yellow fever virus.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody does not cross-react with other flaviviruses.
Antibody reactivity can be assayed by hemagglutination inhibition, complement fixation and immunofluorescence.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
RPMI 1640 medium containing non-essential amino acids, 20 mM HEPES, 1 mM sodium pyruvate, 2 mM L-glutamine, and 0.02 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15%
Subculturing
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.
Medium Renewal: Two to three times weekly

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype
IgG2a; kappa light chain
Name of Depositor
JJ Schlesinger
Deposited As
mouse (B cell); mouse (myeloma)
References

Schlesinger JJ, et al. Monoclonal antibodies distinguish between wild and vaccine strains of yellow fever virus by neutralization, hemagglutination inhibition and immune precipitation of the virus envelope protein. Virology 125: 8-17, 1983. PubMed: 6187129

Monath TP, et al. Yellow fever monoclonal antibodies: type-specific and cross-reactive determinants identified by immunofluorescence. Am. J. Trop. Med. Hyg. 33: 695-698, 1984. PubMed: 6206738

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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