2C5-6

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價格:

編號:B163675

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

2C5-6

商品貨號

B163675

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: BALB/c (B cell); BALB/c (myeloma)

Applications

It reacts with crystalline cholesterol in a solid-phase enzyme-linked immunosorbent assay.

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Spleen cells were fused with P3X63Ag8 myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against cholesterol

Cellular Products

immunoglobulin; monoclonal antibody; against cholesterol

Comments

Animals were immunized with liposomes containing high amounts of cholesterol and lipid A as an adjuvant.

Spleen cells were fused with P3X63Ag8 myeloma cells.

The antibody detects cholesterol in liposomes containing 71% cholesterol but not with liposomes containing 43% cholesterol. It reacts with crystalline cholesterol in a solid-phase enzyme-linked immunosorbent assay.

The antibody is capable of detecting cholesterol in situ in the membranes of skeletal muscle sections.

A feeder layer of mouse peritoneal macrophages or mouse fibroblasts (see ATCC 56-X, irradiated STO cells) is require to propagate this cell line.

Complete Growth Medium

Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.1 mM hypoxanthine and 0.016 mM thymidine, 80%; fetal bovine serum, 20%

Subculturing

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.

Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Cryopreservation

culture medium 95%; DMSO, 5%

Culture Conditions

Temperature: 37.0°C

Isotype

IgM

Name of Depositor

G Schwartz

Deposited As

mouse (B cell); mouse (myeloma)

U.S. Patent Number

4,885,256

References

Clarke MS, et al. In situ localization of cholesterol in skeletal muscle by use of a monoclonal antibody. J. Appl. Physiol. 89: 731-741, 2000. PubMed: 10926660

Swartz GM Jr., et al. Antibodies to cholesterol. Proc. Natl. Acad. Sci. USA 85: 1902-1906, 1988. PubMed: 3162316

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