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2AB1-IA10
2AB1-IA10
規(guī)格:
價(jià)格:
編號(hào):B163671
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
2AB1-IA10
商品貨號(hào)
B163671
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with laminin and can be used to detect laminin in tissues, and for isolation and quantitation of laminin.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against laminin (basement membrane glycoprotein)
Cellular Products
immunoglobulin; monoclonal antibody; against laminin (basement membrane glycoprotein)
Comments
Animals were immunized with extracellular matrix proteins from M1536-B3 cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with laminin and can be used to detect laminin in tissues, and for isolation and quantitation of laminin.
Complete Growth Medium
Culture Medium: Alpha minimum essential medium (Eagle), 90%; heat-inactivated
horse serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2b
Name of Depositor
University of Pittsburgh
Deposited As
rat (B cell); mouse (myeloma)
U.S. Patent Number
References

Chung AE. Novel hybridoma cell line producing monoclonal antibodies against laminin and a process for the production thereof. US Patent 4,642,293 dated Feb 10 1987

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