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293 c18
293 c18
規(guī)格:
價格:
編號:B163664
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
293 c18
商品貨號
B163664
Organism
Homo sapiens, human
Tissue
kidney
培養(yǎng)基
Cell Type
transformed with adenovirus 5 DNA
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
2 CELLS CONTAIN ADENOVIRUS

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
fetus
Storage Conditions
liquid nitrogen vapor temperature
Derivation
This line is a derivative of 293 (see ATCC CRL-1573) the cells express EBNA1.
Comments
Very high frequencies of transformation are obtained with vectors containing the Epstein-Barr virus (EBV) oriP.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.25 mg/ml G-418, 90%; fetal bovine serum, 10%
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 12,14
D16S539: 9
D5S818: 8,9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19
Name of Depositor
Stanford University
Deposited As
Homo sapiens
References

Murphy AJM, et al. cDNA expression cloning in human cells using the pλDR2 episomal vector system. Methods 4(2):111–131,1992.

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