236L

規(guī)格:

價(jià)格:

編號(hào):B163645

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

236L

商品貨號(hào)

B163645

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Product Format

frozen

培養(yǎng)基

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: BALB/c (B cell); BALB/c (myeloma)

Applications

This antibody is specific for the rat subunit integrin alphaD.

It may be used to inhibit inflammation at the site of a central nervous system injury.

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Animals were immunized with purified rat alphaD subunit.

Spleen cells were fused with NS-1 mouse myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against alphaD subunit of rat beta2 (alphaDbeta2) integrin

Cellular Products

immunoglobulin; monoclonal antibody; against alphaD subunit of rat beta2 (alphaDbeta2) integrin

Comments

Animals were immunized with purified rat alphaD subunit.

Spleen cells were fused with NS-1 mouse myeloma cells.

This antibody is specific for the rat subunit integrin alphaD.

It may be used to inhibit inflammation at the site of a central nervous system injury.

Complete Growth Medium

RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 85%; heat-inactivated fetal bovine serum, 15%

Subculturing

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.

Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Cryopreservation

Culture medium supplemented with an additional 50% heat-inactivated fetal bovine serum and 10% DMSO

Culture Conditions

Temperature: 37.0°C

Isotype

IgG1; kappa light chain

Name of Depositor

ICOS Corporation

Deposited As

mouse (B cell); mouse (myeloma)

U.S. Patent Number

6,251,395

References

Gallatin WM, Van der Vieren M. Methods of inhibiting inflammation at the site of a central nervous system injury with alphaD-specific antibodies. US Patent 6,251,395 dated Jun 6 2001

Mabon PJ, et al. Inhibition of monocyte/macrophage migration to a spinal cord injury site by an antibody to the integrin alphaD: a potential new anti-inflammatory treatment. Exp. Neurol. 166: 52-64, 2000. PubMed: 11031083

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