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2.28 M1 [A2,28M1]
2.28 M1 [A2,28M1]
規(guī)格:
價格:
編號:B163629
品牌:Mingzhoubio

標(biāo)準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
2.28 M1 [A2,28M1]
商品貨號
B163629
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with human peripheral blood leukocytes (HLA phenotype A2,Bw6,Bw48,Bw60,Cw4).
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against HLA A2, A28; cytotoxic
Cellular Products
immunoglobulin; monoclonal antibody; against HLA A2, A28; cytotoxic
Comments
Animals were immunized with human peripheral blood leukocytes (HLA phenotype A2,Bw6,Bw48,Bw60,Cw4).
Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells.
The antibody is cytotoxic for a subpopulation of HLA A2 positive cells and HLA A28 positive cells at an appropriate titer.
The antibody recognizes an epitope at the amino terminal end of the alpha 2 domain of the HLA-A2 molecule.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere
  • 0.05 mM 2-Mercaptoethanol

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 5 X 10 exp5 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgM
Name of Depositor
F Grumet
Deposited As
mouse (B cell); mouse (myeloma)
References

Hogan KT, Brown SL. Localization and characterization of serologic epitopes on HLA-A2. Hum. Immunol. 33: 185-192, 1992. PubMed: 1377666

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