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2.03.7
2.03.7
規格:
價格:
編號:B163628
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
2.03.7
商品貨號
B163628
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody reacts with both merozoites and sporozoites by radioimmunoassay and by immunofluorescence.
The antibody cross reacts with sporozoites of E. necatrix and E. maxima.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Eimeria tenella sporozoites and merozoites
Cellular Products
immunoglobulin; monoclonal antibody; against Eimeria tenella sporozoites and merozoites
Tumorigenic
Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized with E. tenella merozoites.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with both merozoites and sporozoites by radioimmunoassay and by immunofluorescence.
The antibody cross reacts with sporozoites of E. necatrix and E. maxima.
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
    • Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Cryopreservation
    culture medium 95%; DMSO, 5%
    Culture Conditions
    Temperature: 37.0°C
    Isotype
    IgG2a
    Name of Depositor
    American Cyanamid Co.
    Deposited As
    mouse (B cell); mouse (myeloma)
    U.S. Patent Number
    References

    Thammana P, et al. Antigens and monoclonal antibodies reactive against merozoites of Eimeria spp.. US Patent 4,650,676 dated Mar 17 1987

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