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1H11
1H11
規格:
價格:
編號:B163626
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
1H11
商品貨號
B163626
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with a conjugate of keyhole limpet hemocyanin (KLH) and a telopeptide having a 3-hydroxypyridinium crosslink derived from the amino terminal domain of bone type I collagen.
The antibody can be used to detect and quantitate degradation products of bone type I collagen in body fluids.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with a conjugate of keyhole limpet hemocyanin (KLH) and a telopeptide having a 3-hydroxypyridinium crosslink derived from the amino terminal domain of bone type I collagen.
Spleen cells were fused with mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against bone type 1 collagen amino terminal telopeptide
Cellular Products
immunoglobulin; monoclonal antibody; against bone type 1 collagen amino terminal telopeptide
Comments
Animals were immunized with a conjugate of keyhole limpet hemocyanin (KLH) and a telopeptide having a 3-hydroxypyridinium crosslink derived from the amino terminal domain of bone type I collagen.
Spleen cells were fused with mouse myeloma cells.
The antibody can be used to detect and quantitate degradation products of bone type I collagen in body fluids.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1
Name of Depositor
Washington Research Foundation
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Eyre DR. Hybridoma cell line producing an antibody to type-I collagen amino-terminal telopeptide. US Patent 5,300,434 dated Apr 5 1994

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