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1939-3G5

規格:

價格:

編號:B163609

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

1939-3G5

商品貨號

B163609

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against Treponema pallidum

Cellular Products

immunoglobulin; monoclonal antibody; against Treponema pallidum

Comments

Animals were immunized with T. pallidum.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody does not react with T. phagedenis.

Complete Growth Medium

Culture Medium: Modified Dulbecco's medium (see below), 90%; fetal bovine
serum, 10%.
DME w/HEPES (10mM) 100 mL
L-Glutamine (100x) 1 mL
Solution I 1 mL
Non-essential amino acids (100x) 1 mL
NCTC 135 10 mL
Fetal Bovine Serum 12 mL
Solution I (100x)
1. 1320 mg oxalacetic acid (100 mM, MW 132).
2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to
100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
aliquot and store frozen.

Subculturing

Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Isotype

IgM

Name of Depositor

Univ. Texas System, Board of Regents

U.S. Patent Number

4,514,498

References

Kettman JR, Norgard MV. Hybrid cell lines producing monoclonal antibodies directed against Treponema. US Patent 4,514,498 dated Apr 30 1985

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