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187.1
187.1
規格:
價格:
編號:B163608
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
187.1
商品貨號
B163608
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
mixed suspension-adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse kappa light chain
Cellular Products
immunoglobulin; monoclonal antibody; against mouse kappa light chain
Comments
Animals were immunized with MPC-11 immunoglobulin.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
    • Subculturing
    Medium Renewal: Every 2 to 3 days
    Subculture by scraping attached cells and transferring with floating cells to a new flask. Start cultures at 2 X 10 (5) cells/ml and maintain between 1 X 10 (5) and 1 X 10 (6) cells/ml.
    Cryopreservation
    Freeze medium: complete growth medium, 95%; DMSO, 5%
    Isotype
    IgG1
    Name of Depositor
    MD Scharff
    Deposited As
    rat (B cell); mouse (myeloma)
    References

    Brumeanu TD, et al. Immunogenicity of a contiguous T-B synthetic epitope of the A/PR/8/34 influenza virus. J. Virol. 71: 5473-5480, 1997. PubMed: 9188620

    Yelton DE, et al. Use of monoclonal anti-mouse immunoglobulin to detect mouse antibodies. Hybridoma 1: 5-11, 1981. PubMed: 6821395

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