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171-4
171-4
規(guī)格:
價(jià)格:
編號(hào):B163601
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
171-4
商品貨號(hào)
B163601
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized a synthetic peptide corresponding to amino acids 257 - 271 of the HLA heavy chain.
The antibody reacts with a conserved epitope in the apha 3 domain of the HLA heavy chain.
In Western blots, the antibody reacts with all HLA-A and HLA-B heavy chains.
It is useful for quantitation of HLA-A and HLA-B antigen expression by immunoblotting.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against HLA heavy chain
Cellular Products
immunoglobulin; monoclonal antibody; against HLA heavy chain
Comments
Animals were immunized a synthetic peptide corresponding to amino acids 257 - 271 of the HLA heavy chain.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with a conserved epitope in the apha 3 domain of the HLA heavy chain.
In Western blots, the antibody reacts with all HLA-A and HLA-B heavy chains.
It does not bind well to native HLA antigens.
It is useful for quantitation of HLA-A and HLA-B antigen expression by immunoblotting.
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgG2a; kappa light chain
Name of Depositor
K Kao
Deposited As
mouse (B cell); mouse (myeloma)
References

Kao KJ, Riley WJ. Genetic predetermination of quantitative expression of HLA antigens in platelets and mononuclear leukocytes. Hum. Immunol. 38: 243-250, 1993. PubMed: 8138419

Shieh DC, Kao KJ. A combined approach for quantitation of each specific HLA-A or -B antigen expressed on cells. J. Immunol. Methods 184: 169-176, 1995. PubMed: 7658021

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