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17/14

規格:

價格:

編號:B163599

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

17/14

商品貨號

B163599

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Spleen cells were fused with NS-1 myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against theophylline

Cellular Products

immunoglobulin; monoclonal antibody; against theophylline

Tumorigenic

Yes

Effects

Yes, forms ascites in pristane primed BALB/c mice

Comments

Animals were immunized with theophylline-8-KLH.

Spleen cells were fused with NS-1 myeloma cells.

Complete Growth Medium

Culture medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%;
fetal bovine serum, 20%.

Subculturing

Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Isotype

IgG1; kappa light chain

Name of Depositor

E.I. du Pont de Nemours & Co.

Deposited As

mouse (B cell); mouse (myeloma)

U.S. Patent Number

4,524,025

References

Geltosky JE. Hybridoma cell lines and monoclonal antibodies to theophylline. US Patent 4,524,025 dated Jun 18 1985

Geltosky JE. Hybridoma cell lines and monoclonal antibodies to theophylline. US Patent 4,521,510 dated Jun 4 1985

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