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16M3F10
16M3F10
規(guī)格:
價(jià)格:
編號:B163598
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
16M3F10
商品貨號
B163598
Organism
human (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Human peripheral blood mononuclear cells (enriched for B cells) were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against diphtheria toxin
Cellular Products
immunoglobulin; monoclonal antibody; against diphtheria toxin
Comments
Human peripheral blood mononuclear cells (enriched for B cells) were fused with P3X63Ag8.653 myeloma cells.
Complete Growth Medium
RPMI 1640 medium with 2 mM L-glutamine, 1 mM sodium pyruvate, 5 mcg/ml insulin and 5 mcg/ml transferrin, 85%; fetal bovine serum, 15%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG
Name of Depositor
University of Rochester
Deposited As
human (B cell); mouse (myeloma)
U.S. Patent Number
References

Insel RA. Human monoclonal antibodies against bacterial toxins. US Patent 4,689,299 dated Aug 25 1987

Gigliotti F, et al. Reproducible production of protective human monoclonal antibodies by fusion of peripheral blood lymphocytes with a mouse myeloma cell line. J. Infect. Dis. 149: 43-47, 1984. PubMed: 6607295

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