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162-21.2
162-21.2
規格:
價格:
編號:B163592
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
162-21.2
商品貨號
B163592
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Choriocarcinoma
Applications
The antibody reacts with an antigen (Trop-1) present on trophoblast cells and on human choriocarcinomas.
The antigen has also been detected on 4 of 4 human choriocarcinoma cell lines tested, on a fibrosarcoma cell line (HT1080C), on an erythroleukemia cell line (K562) and on the majority of human malignant epithelial cells and fetal epithelial precursors.
No staining was detected on peripheral blood cells.
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Trop-1 antigen
Cellular Products
immunoglobulin; monoclonal antibody; against Trop-1 antigen
Comments
Animals were immunized with the human choriocarcinoma cell line BeWo.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with an antigen (Trop-1) present on trophoblast cells and on human choriocarcinomas.
The antigen has also been detected on 4 of 4 human choriocarcinoma cell lines tested, on a fibrosarcoma cell line (HT1080C), on an erythroleukemia cell line (K562) and on the majority of human malignant epithelial cells and fetal epithelial precursors.
The gene encoding the trop-1 antigen has been cloned.
No staining was detected on peripheral blood cells.
Complete Growth Medium
Culture Medium: RPMI 1640 medium, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2a
Name of Depositor
LA Herzenberg
Deposited As
mouse (B cell); mouse (myeloma)
References

Lipinski M, et al. Human trophoblast cell-surface antigens defined by monoclonal antibodies. Proc. Natl. Acad. Sci. USA 78: 5147-5150, 1981. PubMed: 7029529

Strnad J, et al. Molecular cloning and characterization of a human adenocarcinoma/epithelial cell surface antigen complementary DNA. Cancer Res. 49: 314-317, 1989. PubMed: 2463074

Klein CE, et al. Expression of 38-kD cell-surface glycoprotein in transformed human keratinocyte cell lines, basal cell carcinomas, and epithelial germs. J. Invest. Dermatol. 95: 74-82, 1990. PubMed: 2366004

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