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145-2C11
145-2C11
規格:
價格:
編號:B163576
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
145-2C11
商品貨號
B163576
Organism
Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)
Tissue
Spleen, blood
培養基
Cell Type
Hybridoma: B lymphocyte
Product Format
frozen
Morphology
Lymphoblast-like
Culture Properties
Suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications

It reacts with all mature T cells and can both activate and inhibit T cell function. The antibody is specific for a 25000 dalton protein component (CD3 epsilon) of the antigen specific T cell receptor. The antibody reacts with the murine T cell receptor (CD3 - T3) complex.

The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.

The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.
Storage Conditions
liquid nitrogen vapor phase
Derivation

Animals were immunized with the BM10-37 mouse cytotoxic T lymphocyte (CTL) cell clone (anti H-2 Kb). Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Genes Expressed
immunoglobulin; monoclonal antibody; against mouse CD3
Cellular Products
immunoglobulin; monoclonal antibody; against mouse CD3
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Isotype
IgG , IgG
Name of Depositor
JA Bluestone
Deposited As
hamster, Armenian (B cell); mouse (myeloma)
References

Leo O, et al. Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc. Natl. Acad. Sci. USA 84: 1374-1378, 1987. PubMed: 2950524

Kayagaki N, et al. Polymorphism of murine Fas ligand that affects the biological activity. Proc. Natl. Acad. Sci. USA 94: 3914-3919, 1997. PubMed: 9108079

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