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132C4A/4
132C4A/4
規格:
價格:
編號:B163563
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
132C4A/4
商品貨號
B163563
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against surface (cercariae) glycoprotein on Schistosoma mansoni
Cellular Products
immunoglobulin; monoclonal antibody; against surface (cercariae) glycoprotein on Schistosoma mansoni
Comments
Animals were immunized with cercariae glycoprotein from Schistosoma mansoni.
Spleen cells were fused with P3X63Ag8 myeloma cells.
Complete Growth Medium
Culture Medium: Modified Dulbecco's medium (see below) with HAT, 90%; fetal
bovine serum, 10%.
DME w/HEPES (10mM) 100 mL
L-Glutamine (100x) 1 mL
Solution I 1 mL
Non-essential amino acids (100x) 1 mL
NCTC 135 10 mL
Fetal Bovine Serum 12 mL
Solution I (100x)
1. 1320 mg oxalacetic acid (100 mM, MW 132).
2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to
100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
aliquot and store frozen.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 40%; fetal bovine serum, 50%; DMSO, 10%
Isotype
IgG1
Name of Depositor
Johns Hopkins University
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Strand M. Diagnosis and treatment of fluke infections with monoclonal antibodies. US Patent 4,416,866 dated Nov 22 1983

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