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123-10
123-10
規格:
價格:
編號:B163553
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
123-10
商品貨號
B163553
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for the alpha subunit and recognizes a determinant present only on the denatured enzyme.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against DNA polymerase III holoenzyme from Escherichia coli (E. coli)
Cellular Products
immunoglobulin; monoclonal antibody; against DNA polymerase III holoenzyme from Escherichia coli (E. coli)
Comments
Mice were immunized with E. coli DNA polymerase III' containing the alpha, epsilon, theta and tau subunits.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody is specific for the alpha subunit and recognizes a determinant present only on the denatured enzyme.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Iscove's modified Dulbecco's medium, 85%; fetal bovine serum, 15%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgM
Name of Depositor
M Franden, CS McHenry
Deposited As
mouse (B cell); mouse (myeloma)
References

Wu YH, et al. Monoclonal antibodies specific for the alpha subunit of the Escherichia coli DNA polymerase III holoenzyme. J. Biol. Chem. 259: 12117-12122, 1984. PubMed: 6090455

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