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12/49

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價格:

編號:B163551

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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甘油

平板

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產品名稱

12/49

商品貨號

B163551

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

BALB/c (B cell); BALB/c (myeloma)

Applications

The antibody cross-reacts with human H-Y.

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.

Clinical Data

Female BALB/c mice were immunized with lymphocytes from male BALB/c mice.

Genes Expressed

immunoglobulin; monoclonal antibody; against H-Y antigen

Cellular Products

immunoglobulin; monoclonal antibody; against H-Y antigen

Tumorigenic

Yes

Effects

Yes, forms ascites in BALB/c mice

Comments

Female BALB/c mice were immunized with lymphocytes from male BALB/c mice.

Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.

The antibody cross-reacts with human H-Y.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Cryopreservation

culture medium 95%; DMSO, 5%

Culture Conditions

Temperature: 37.0°C

Isotype

IgM; kappa light chain

Name of Depositor

Sloan-Kettering Institute for Cancer Research

Deposited As

mouse (B cell); mouse (myeloma)

U.S. Patent Number

4,680,258

References

Koo GC, et al. Process for sex determination in man by use of monoclonal antibodies to the H-Y antigen. US Patent 4,680,258 dated Jul 14 1987

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