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12/44
12/44
規(guī)格:
價(jià)格:
編號(hào):B163550
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
12/44
商品貨號(hào)
B163550
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody is cytotoxic for BALB/c male lymph node cells and cross-reacts with human H-Y.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
Clinical Data
Female BALB/c mice were immunized with lymphocytes from male BALB/c mice.
The antibody is cytotoxic for BALB/c male lymph node cells and cross-reacts with human H-Y.
Genes Expressed
immunoglobulin; monoclonal antibody; against H-Y antigen
Cellular Products
immunoglobulin; monoclonal antibody; against H-Y antigen
Tumorigenic
Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Female BALB/c mice were immunized with lymphocytes from male BALB/c mice.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
The antibody is cytotoxic for BALB/c male lymph node cells and cross-reacts with human H-Y.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgM; kappa light chain
Name of Depositor
Sloan-Kettering Institute for Cancer Research
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Koo GC, et al. Process for sex determination in man by use of monoclonal antibodies to the H-Y antigen. US Patent 4,680,258 dated Jul 14 1987

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