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11P0-1
11P0-1
規格:
價格:
編號:B163548
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
11P0-1
商品貨號
B163548
Organism
Mus musculus, mouse
Cell Type
mast cell; Abelson murine leukemia virus transforme
培養基
Product Format
frozen
Morphology
mast cell
Culture Properties
suspension
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
11 days gestation
Applications
The 11P0-1 cell line was established by transformation of placental cells from an 11 day mouse embryo with A-MuLV.
Derivation
The 11P0-1 cell line was established by transformation of placental cells from an 11 day mouse embryo with A-MuLV.
Receptor Expression
FcERI (Fc of IgE)
Comments
The 11P0-1 cell line was established by transformation of placental cells from an 11 day mouse embryo with A-MuLV.
The cells are surface immunoglobulin positive, B23.1 positive, B220 positive and possess receptors for IgE.
This line represents a transformed stage of a very early hematopoietic precursor.
Complete Growth Medium
RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp4 cells/ml and maintain between 1 X 10 exp4 and 5 X 10 exp5 cells/ml. Do not allow the cell density to exceed 5 X 10 exp5 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor
EJ Siden
Deposited As
Mus musculus
References

Siegel ML, et al. Isolation and characterization of Abelson murine leukemia virus- transformed mast cell lines from midgestation embryonic placenta. Eur. J. Immunol. 15: 1136-1141, 1985. PubMed: 2998805

Siden EJ. Regulated expression of germline antigen receptor genes in mast cell lines from the murine embryo. J. Immunol. 150: 4427-4437, 1993. PubMed: 7683318

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