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1116NS-3d
1116NS-3d
規(guī)格:
價格:
編號:B163540
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
1116NS-3d
商品貨號
B163540
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Adenocarcinoma
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against carcinoembryonic antigen (CEA)
Cellular Products
immunoglobulin; monoclonal antibody; against carcinoembryonic antigen (CEA)
Comments
Animals were immunized with a human colonic adenocarcinoma (SW1116, ATCC CCL-233).
Spleen cells were fused with P3X63Ag8 myeloma cells.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgM
Name of Depositor
Wistar Institute
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Koprowski H, et al. Monoclonal hybridoma antibody specific for high molecular weight carcinoembryonic antigen. US Patent 4,349,528 dated Sep 14 1982

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