Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Note that activities with high potential for aerosol production require BSL 3 facilities and practices.
Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 rpm (750 x g) for one hour. Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 µg /mL cycloheximide. Incubate at 36°C for 3 days.
Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus.
Wang SP, et al. Three new immunologic types of trachoma-inclusion conjunctivitis organisms. J. Immunol. 110: 873-879, 1973. PubMed: 4631875
Fong CK, et al. Re-examination of the McCoy cell line for confirmation of its mouse origin: karyotyping, electron microscopy and reverse transcriptase assay for endogenous retrovirus. Clin Diagn Virol 2(2): 5-103, 1994. PubMed: 15566757
