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Vaccinia virus
Vaccinia virus
規格:
價格:
編號:B163302
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Vaccinia virus
商品貨號
B163302
Classification
Poxviridae, Orthopoxvirus
Deposited As
Vaccinia virus, Orthopoxvirus
培養基
Agent
Vaccinia virus
Strain
IHD-W
Applications
IHD-W is the strain used by Dales to develop a large group of ts mutants in the 1970s.
The host range includes most mammalian cells and tissues.
Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no.
IHD-W is the strain used by Dales to develop a large group of ts mutants in the 1970s.
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
The IHD-W subtype was derived in Japan from IHD-J strain of vaccinia by selection for the ability to induce distinct clear plaques in culture.
Product Format
frozen
Storage Conditions
-70°C
Comments
IHD-W is the strain used by Dales to develop a large group of ts mutants in the 1970s. IHD-W differs from the IHD-J parent strain, in that IHD-W is unable to induce an active hemagglutinin in the plasma membrane and does elicit polykaryoctosis.
The host range includes most mammalian cells and tissues.
Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4).
IHD-W is the strain used by Dales to develop a large group of ts mutants in the 1970s. IHD-W differs from IHD-J in that IHD-W is HA(-) and IHD-J is HA(+).
-70C
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
CPE
Recommended Host
L2 cells (ATCC CCL-149); chicken embryo fibroblasts

Host of Choice: L2 and CEF

Host Range: most mammalian cells and tissues

most mammalian cells and tissues
Growth Conditions
Duration: 1-5 days at 37C
Cryopreservation
Storage temperature: -70C
Name of Depositor
S Dales
Special Collection
NCRR Contract
Source
The IHD-W subtype was derived in Japan from IHD-J strain of vaccinia by selection for the ability to induce distinct clear plaques in culture.
References

Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807

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