Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Temperature: 35°C
Incubation: 20 to 28 days at 35°C, a 5% CO2 in air atmosphere is recommended.
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 70% - 80% confluent (not 100% confluent).
Binn LN, et al. Primary isolation and serial passage of hepatitis A virus strains in primate cell cultures. J. Clin. Microbiol. 20: 28-33, 1984. PubMed: 6086708
