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Encephalomyocarditis virus

規格:

貨期:

編號:B163074

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

Encephalomyocarditis virus

商品貨號

B163074

Classification

Picornaviridae, Cardiovirus

Agent

Encephalomyocarditis virus

Strain

M-variant (TC passaged)

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format

frozen

Storage Conditions

-70°C or colder

Comments

ATCC^® VR-1479™ (M-varient) is a cell culture passaged linage of the M-varient (ATCC^® VR-1314™) reported to induce diabetes in selected strains of mice such as Balb/cBy and DBA/2 mice. Note that virus loses diabetagenic properties upon multiple passage in cell culture.

Effect on Host

Rounding and sloughing with general degeneration

Recommended Host

NCTC Clone 929 (ATCC^® CCL-1™)

Growth Conditions

Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 75-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm²), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1 hour at 37°C in a humidified 5% CO₂ atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 4-10 days

Name of Depositor

ATCC

Special Collection

NCRR Contract

Source

ATCC^® VR-1479™ was created by serially passaging ATCC^® VR-1314™ two times onto NCTC clone 929 (L-929) cells, ATCC^® CCL-1™. The original strain was isolated from the heart of mice inoculated by peripheral route with EMC of swine.

References

Craighead JE. Pathogenicity of the M and E variants of the encephalomyocarditis (EMC) virus. I. Myocardiotropic and neurotropic properties. Am. J. Pathol. 48: 333-345, 1966. PubMed: 4285783

B208468：Encephalomyocarditis virus

B181716：Encephalomyocarditis virus (Cell culture adapted)

B176016：Encephalomyocarditis virus (Murine) (tissue culture adapted)

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