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Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Parvovirus h-1, complete genome
Temperature: 37°C
Recommendations for Infection: Plant cells 24-48 hours in advance and infect when cultures are about 70% confluent. Remove medium and inoculate with a small volume of virus diluted to provide a MOI of about 0.1. Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 5 days at 37°C temperature in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Toolan H, Ledinko N. Growth and cytopathogenicity of H-viruses in human and simian cell cultures. Nature 208: 812-813, 1965. Pubmed: 4958078
Karasaki S. Size and ultrastructure of the H-viruses as determined with the use of specific antibodies. J Ultrastruct Res 16(1): 109-122, 1966. Pubmed: 4959542
Proc. Am. Assoc. Cancer Res. 5: 64, 1964.
Rhodes SL, Paradiso PR. Parvovirus genome: nucleotide sequence of H-1 and mapping of its genes by hybrid-arrested translation. J Virol 45(1): 173-184, 1983.
Nucleotide (GenBank) : X01457.1 Parvovirus h-1, complete genome
