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Murid herpesvirus 1
Murid herpesvirus 1
規(guī)格:
貨期:
編號:B162738
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Murid herpesvirus 1
商品貨號
B162738
Classification
Herpesviridae, Muromegalovirus
Deposited As
Mouse salivary gland virus
Agent
Murid herpesvirus 1
Strain
Smith MSGV
Positive for Mycoplasma
No
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen 1 mL per vial
Storage Conditions
Vapor phase of liquid nitrogen
Comments
Virus primarily cell-associated. Cryopreserve virus in 8% DMSO (ATCC® 4-X™).
Effect on Host
CPE, cell rounding and degeneration
Recommended Host
SC-1 cells (ATCC® CRL-1404™)
Growth Conditions
Temperature: 37°C
Recommendations For Infection: This product is produced by co-cultivation of virus with fresh host cells. Prepare a bulk cell suspension the day of inoculation. Seed culture vessels at 8 x 104 - 1 x 105 cells per cm2. Calculate the volume of virus needed to achieve an optimal MOI (e.g. 0.01) and then dilute virus in virus growth medium to prepare the virus inoculum. Add virus inoculum to culture vessels. Incubate for 24 hours at 37°C in a humidified 5% CO2 atmosphere. Aspirate virus growth medium to remove any traces of DMSO and then add fresh virus growth medium to cultures. Continue incubation.
Incubation: 2-7 days
Name of Depositor
WP Rowe, National Institute of Allergy and Infectious Diseases
Special Collection
ATCC
Source
Salivary gland tissues from three naturally infected mice. Derived from VR-194™. Treated to remove Mycoplasma hominis at ATCC®.
References

Selgrade MK, et al. Effects of cell source, mouse strain, and immunosuppressive treatment on production of virulent and attenuated murine cytomegalovirus. Infect. Immun. 33: 840-847, 1981. PubMed: 6270000

Smith MG. Propagation of salivary gland virus of the mouse in tissue cultures. Proc. Soc. Exp. Biol. Med. 86: 435-440, 1954. PubMed: 13194679

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