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HL-40

規格:

價格:

編號:B162674

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

HL-40

商品貨號

B162674

Designations

HL-40

GenBank Number

J02778

培養基

Species

Homo sapiens, human

Depositors

K Tryggvason

Vector

Construct size (kb): 8.199999809265137

Insert

DNA: cDNA

Insert lengths(kb): 3.700000047683716

Tissue: placenta

Gene product: laminin, B1 polypeptide [LAMB1]

Alleles: A1, A2

Insert Size (kb)

3.700

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

The insert contains PstI sites at nucleotides 3460, 4327, and 4893 relative to the 5' end of the complete cDNA sequence.

Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 3.8; BamHI--8.2; PstI--5.5, 1.2, 0.9, 0.6; SalI--7.4, 0.8; HindIII--8.2.

This insert extends from nucleotide 1402 through about nucleotide 5100 of the 5.5 kb cDNA.

HL-40 (ATCC 65008, 65009) and HL-42 (ATCC 65014, 65015) overlap.

Enzyme(s) not detecting polymorphism: EcoRI, SphI, BamHI, HindIII, MspI, TaqI, PstI, HaeIII, RsaI, HinfI (in 20 unrelated Finnish individuals).

References

Ikonen J, et al. A Hpa I polymorphism in the human laminin B1 chain gene on 7q22. Nucleic Acids Res. 17: 473, 1989. PubMed: 2563160

Pikkarainen T, et al. Human laminin B1 chain. A multidomain protein with gene (LAMB1) locus in the q22 region of chromosome 7. J. Biol. Chem. 262: 10454-10462, 1987. PubMed: 3611077

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