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RBL-1
RBL-1
規(guī)格:
價格:
編號:B162572
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
RBL-1
商品貨號
B162572
Organism
Rattus norvegicus, rat
Tissue
peripheral blood
培養(yǎng)基
Cell Type
chemically-induced basophil
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
leukemia
Strain
Wistar
Applications
This cell line is a suitable transfection host.
Storage Conditions
liquid nitrogen vapor phase
Receptor Expression
FcERI (Fc of IgE)
Comments
This cell line exhibits various characteristics of basophil differentiation including surface receptors for IgE. It was shown to not release histamine by an IgE mediated system. RefBarsumian EL, et al. IgE-induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clones. Eur. J. Immunol. 11: 317-323, 1981. PubMed: 6166481
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
 Cultures can be maintained by the addition or replacement of fresh medium. Start cultures at 2 x 105 viable cells/mL and maintain between 1 x 105 cells/mL and 1 x 106 /mL.
Medium Renewal: Add medium as cell density increases
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Name of Depositor
H Metzger, C Isersky
Deposited As
Rattus sp.
References

Kulczycki A Jr., et al. The interaction of IgE with rat basophilic leukemia cells. I. Evidence for specific binding of IgE. J. Exp. Med. 139: 600-616, 1974. PubMed: 4812630

Barsumian EL, et al. IgE-induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clones. Eur. J. Immunol. 11: 317-323, 1981. PubMed: 6166481

Eccleston E, et al. Basophilic leukaemia in the albino rat and a demonstration of the basopoietin. Nat. New Biol. 244: 73-76, 1973.

Cross References

Nucleotide (GenBank) : NM_031580 Rattus norvegicus glucose regulated protein, 58 kDa (Grp58), mRNA.

Nucleotide (GenBank) : X12355 Rat mRNA for phosphoinositide-specific phospholipase C form-I (PI-PLC I).

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