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pGEM-8

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編號:B162556

品牌:Mingzhoubio

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產品名稱

pGEM-8

商品貨號

B162556

Designations

pGEM-8

GenBank Number

J03528

培養基

Species

Homo sapiens, human

Depositors

WS Sly

Vector

Construct size (kb): 12.0

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-2
Intact vector size: 2.870
Type of vector: plasmid
Vector end: SalI
Vector end: XbaI
Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
MCS: EcoRI...HindIII, ->, 10-60
promoter for in vitro transcription: T7, <-, 65-87
replicon: pMB1
marker(s): ampR, <-, 1098-1958
promoter for in vitro transcription: SP6, ->, 2852-2869
Cross references: DNA Seq. Acc.: X65301

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IGF2R
Genomic copy number: unique
Gene name: insulin-like growth factor 2 receptor
Contains complete coding sequence?: Y
Chromosome: 6; Localization: 6 q25.3
Tissue: placenta
Type of DNA: cDNA
Insert 5' end: SalI
Insert 3' end: XbaI
Insert size (kb): 9.1
Cross references: DNA Seq. Acc.: J03528

Insert lengths(kb): 9.100000381469727

Tissue: placenta

Gene product: insulin-like growth factor 2 receptor( insulin-like growth factor II receptor, insulin like growth factor II (IGF II) receptor, mannose-6-phosphate receptor, cation-independent) [IGF2R]

Alleles: A1, A1

Insert Size (kb)

9.100

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--6.6, 4.0, 1.8; EcoRI--8.0, 3.7; HindIII--5.0, 4.0, 1.8, 1.5; SalI--9.0, 2.7; SalI/XhoI--9.0, 2.7.

The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--0.48, 8.40; HindIII--0.72, 2.24, 3.94; BamHI--2.81, 2.96, 3.34, 6.91.

At the 3' end of the insert, the construct includes sequence from the vector pSLV: XbaI, SmaI, SacI, and BamHI sites, and the SV40 poly(A) polyadenylation signal (to the SalI site).

References

Oshima A, et al. The human cation-independent mannose 6-phosphate receptor. Cloning and sequence of the full-length cDNA and expression of functional receptor in COS cells. J. Biol. Chem. 263: 2553-2562, 1988. PubMed: 2963003

William S Sly, personal communication

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