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HK-PEG-1 [formerly PEG 1-6]
HK-PEG-1 [formerly PEG 1-6]
規(guī)格:
價(jià)格:
編號(hào):B162543
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
HK-PEG-1 [formerly PEG 1-6]
商品貨號(hào)
B162543
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue
spleen
培養(yǎng)基
Cell Type
hybridoma: B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Influenza
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
The line was produced by fusing P3X63Ag8 myeloma cells with spleen cells from BALB/c mice that had been immunized with influenza virus (A.PR/8/34).
Tumorigenic
Yes
Effects
Yes, in BALB/c mice
Comments
The hybridoma cell line HK-PEG-1 secretes a mouse monoclonal antibody (IgG3) that reacts with influenza virus. 
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 6 to 8 x 104 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL.
Medium Renewal: Add fresh medium (depending on cell density) every 2-3 days.
Cryopreservation
Complete growth medium supplemented with 10% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions

Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%

Isotype
mouse IgG3
Name of Depositor
WU Gerhard
U.S. Patent Number
References

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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