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B11F7
B11F7
規格:
價格:
編號:B162519
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
B11F7
商品貨號
B162519
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
B lymphocyte; hybridom
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts well with denatured MAG, and is excellent for western blots and immunostaining of fixed tissue.
The B11F7 monoclonal antibody recognizes a polypeptide epitope and is highly specific for MAG of most species.
Storage Conditions
liquid nitrogen vapor phase
Images
Derivation
Animals were immunized with human myelin-associated glycoprotein (MAG) isolated from central nervous system myelin. Spleen cells from immunized mice were fused with P3X63Ag8.653 mouse myeloma cells and the resulting MAG antibody secreting hybrids were identified using the ELISA assay. The B11F7 monoclonal antibody recognizes a polypeptide epitope and is highly specific for MAG of most species. Immunocytochemical staining of adult human spinal cord with the antibody resulted in periaxonal staining of myelin sheaths. The antibody reacts well with denatured MAG, and is excellent for western blots and immunostaining of fixed tissue. However, it does not bind to native MAG, so it does not immunostain live cells or block function.
Genes Expressed
immunoglobulin; monoclonal antibody; against protein backbone of human and rat myelin-associated glycoprotein (MAG)
Cellular Products
immunoglobulin; monoclonal antibody; against protein backbone of human and rat myelin-associated glycoprotein (MAG)
Comments
Animals were immunized with human myelin-associated glycoprotein (MAG) isolated from central nervous system myelin. Spleen cells from immunized mice were fused with P3X63Ag8.653 mouse myeloma cells and the resulting MAG antibody secreting hybrids were identified using the ELISA assay. The B11F7 monoclonal antibody recognizes a polypeptide epitope and is highly specific for MAG of most species. Immunocytochemical staining of adult human spinal cord with the antibody resulted in periaxonal staining of myelin sheaths. The antibody reacts well with denatured MAG, and is excellent for western blots and immunostaining of fixed tissue. However, it does not bind to native MAG, so it does not immunostain live cells or block function.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 104 to 1 x 105 viable cells/ mL. Maintain cultures at a cell concentration between 5 x 105 and 1 x 106 cells/mL.

Medium Renewal: Two to three times weekly

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype
IgG2b, kappa
Name of Depositor
RH Quarles
Year of Origin
1984
References

Doberson MJ, et al. Generation and characterization of mouse monoclonal antibodies to the myelin-associated glycoprotein (MAG). Neurochem. Res.;10(4):499-513, 1985. PubMed: 2582290

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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