The vector can be used whenever it is necessary to maintain two plasmids within the same cell.
Comments
Restriction digests of the clone give the following sizes (kb): BglI--2.9, 1.1; AvaII--3.4, 0.6; HindIII--4.0.
The vector can be used whenever it is necessary to maintain two plasmids within the same cell.
ATCC 87497 and 87498 differ in multiple cloning sites.
Media
ATCC® Medium 1639: LB medium (ATCC medium 1065) with 50 mcg/ml spectinomycin
Growth Conditions
Temperature: 37.0°C
References
Manen D, et al. A sensitive reporter gene system using bacterial luciferase based on a series of plasmid cloning vectors compatible with derivatives of pBR322. Gene 186: 197-200, 1997. PubMed: 9074496
