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pRY121 plasmid in <em>E. coli</em>

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價格:

編號:B162052

品牌:Mingzhoubio

標準菌株

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DNA

RNA

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凍干粉

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產品名稱

pRY121 plasmid in E. coli

商品貨號

B162052

Designations

pRY121 plasmid in E. coli

Species

Saccharomyces cerevisiae Meyen ex E.C. Hansen

培養基

Depositors

JM Becker

Vector

Total size of construct (kb): 11.50
Markers: ampR, lacZ+, URA3
Excise insert: SalI/BamHI

Vector: pLG67OZ
Vector type: plasmid
Vector size (kb):
Markers: ampR

Insert

Insert size (kb): 0.7
Gene: Galactose promoter region
Source: Saccharomyces cerevisiae
Insert ends: SalI/BamHI

Insert Size (kb)

0.700

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Frozen glycerol stock of Escherichia coli GC10 containing the plasmid.

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--3.2(doublet), 2.8, 2.2; HindIII--6.2, 2.15, 1.45, 1.05; SalI--11.5.

Contains S. cerevisiae GAL1 promoter region (from about 1 kb upstream of the GAL1 transcription start site to amino acid 33) fused to the bacterial lacZ gene.

Classification

Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae

References

West RW Jr., et al. Saccharomyces cerevisiae GAL1-GAL10 divergent promoter region: location and function of the upstream activating sequence UASG. Mol. Cell. Biol. 4: 2467-2478, 1984. PubMed: 6392852

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