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pRLalpha2M/29J
pRLalpha2M/29J
規(guī)格:
價(jià)格:
編號(hào):B161972
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pRLalpha2M/29J
商品貨號(hào)
B161972
Designations
pRLalpha2M/29J
GenBank Number

J02635

培養(yǎng)基
Species
Rattus norvegicus, rat
Depositors
GH Fey
Vector
Construct size (kb): 7.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 4.599999904632568
Tissue: liver, male, acute phase
Gene product: alpha-2-macroglobulin [A2M]
Insert Size (kb)
4.600
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
The vector does not have convenient restriction sites close to the insert. A BamHI digest yields 3 fragments from the insert, 5' 0.7, 3.54, 0.52 kb 3'. The end fragments contain vector sequences, and the vector band is about 3 kb.
The insert contains the following restriction sites (nucleotide position according to GenBank J02635): BglII--7; BamHI--576, 4116; PvuII--637, 2174, 2536, 3040; HindIII--1440; XbaI--2217.
Restriction digests of the clone give the following sizes (kb): BamHI--3.5, 3.0, 0.6, 0.5; EcoRI--4.6, 2.6, 0.4; PstI--4.3, 2.0, 1.3; HindIII--5.6, 2.0; SalI--7.6.
Contains the complete coding sequence (nt 64-4482).
References

Gehring MR, et al. Sequence of rat liver alpha2-macroglobulin and acute phase control of its messenger RNA. J. Biol. Chem. 262: 446-454, 1987. PubMed: 2432068

Georg H Fey, personal communication

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