Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Note: The culture flasks should be treated with 0.1mL/cm2 of flask surface area with 15 µg/mL poly-L-lysine (Sigma Cat. No. P-9155 or equivalent) for at least 2 hours at 37°C. Remove solution and rinse one time with DPBS and allow flask to air dry uncapped and standing upright in a biological cabinet for about 30 minutes before introducing cells.Toda K, et al. Biochemical and cellular properties of three immortalized Schwann cell lines expressing different levels of the myelin-associated glycoprotein. J. Neurochem. 63(5):1646-1657, 1994. PubMed: 7523597
Goda S, et al. Expression of the myelin-associated glycoprotein in cultures of immortalized Schwann cells. J. Neurochem. 56(4):1354-1361, 1991. PubMed: 1705958
Sasagasako N, et al. Myelin gene expression in immortalized Schwann cells: relationship to cell density and proliferation. J. Neurochem. 66(4): 1432-1439, 1996. PubMed: 8627295
