Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Temperature: 37°C
Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Subramaniam K, et al. Genomic Sequences of Epizootic Hemorrhagic Disease Viruses Isolated from Florida White-Tailed Deer. Genome Announc. 5(43): e01174-17, 2017. Pubmed: 29074661
Sun F, et al. Molecular typing of epizootic hemorrhagic disease virus serotypes by one-step multiplex RT-PCR. J Wildl Dis 50(3): 639-44, 2014. PubMed: 24807175
Nucleotide (GenBank) : MF688826.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP1 protein (VP1) gene, complete cds
Nucleotide (GenBank) : MF688827.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP2 protein (VP2) gene, complete cds.
Nucleotide (GenBank) : MF688828.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP3 protein (VP3) gene, complete cds
Nucleotide (GenBank) : MF688829.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP4 protein (VP4) gene, complete cds
Nucleotide (GenBank) : MF688830.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP5 protein (VP5) gene, complete cds
Nucleotide (GenBank) : MF688831.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP6 protein (VP6) gene, complete cds
Nucleotide (GenBank) : MF688832.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP7 protein (VP7) gene, complete cds
Nucleotide (GenBank) : MF688833.1 Epizootic hemorrhagic disease virus 1 isolate OV202 NS1 protein (NS1) gene, complete cds
Nucleotide (GenBank) : MF688834.1 Epizootic hemorrhagic disease virus 1 isolate OV202 NS2 protein (NS2) gene, complete cds
Nucleotide (GenBank) : MF688835.1 Epizootic hemorrhagic disease virus 1 isolate OV202 NS3 protein (NS3) gene, complete cds
