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NG108-15 [108CC15]

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價格:

編號:B161362

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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甘油

平板

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產品名稱

NG108-15 [108CC15]

商品貨號

B161362

Organism

Mus musculus (neuroblastoma); Rattus norvegicus (glioma), mouse (neuroblastoma); rat (glioma)

Tissue

brain

培養基

Cell Type

somatic cell hybrid

Product Format

frozen

Morphology

flat; round; 10 to 100 micrometers diameter

Culture Properties

Adherent, but please note: as the culture media becomes acidic these cells begin to detach and grow as a suspension, but they will typically reattach again when fresh medium is added.

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

glioblastoma; neuroblastoma

Applications

This cell line is a suitable transfection host.

Storage Conditions

liquid nitrogen vapor phase

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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Derivation

The line was formed by fusing mouse N18TG2 neuroblastoma cells with rat C6-BU-1 glioma cells in the presence of inactivated Sendai virus.

The NG108-15 cell line, originally named 108CC15, was developed in 1971 by Bernd Hamprecht.

Complete Growth Medium

The base medium for this cell line is Dulbecco's Modified Eagle's Medium (GIBCO/InVitrogen Catalog No.12100-061, DMEM without sodium pyruvate ). To make the complete growth medium, add the following components to the base medium:

0.1 mM hypoxanthine (final conc.)
400 nM aminopterin (final conc.)
0.016 mM thymidine (final conc.)
10% fetal bovine serum (final conc.)
1.5 g/L sodium bicarbonate

Subculturing

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

Name of Depositor

Univ. Texas Southwestern Medical Cntr.

Deposited As

mouse (neuroblastoma); rat (glioma)

U.S. Patent Number

5,728,808

References

Hamprecht B. Structural, electrophysiological, biochemical, and pharmacological properties of neuroblastoma-glioma cell hybrids in cell culture. Int. Rev. Cytol. 49: 99-170, 1977. PubMed: 16829

Hamprecht B, et al. Culture and characteristics of hormone-responsive neuroblastoma X glioma hybrid cells. Methods Enzymol. 109: 316-341, 1985. PubMed: 2985920

Bernd Hamprecht, personal communication

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