通過(guò)將pheP和3個(gè)stlA拷貝整合到大腸桿菌Nissle染色體中，引入誘導(dǎo)型啟動(dòng)子PfnrS，獲得一個(gè)能夠代謝苯丙酮酸的菌株SYNB1618。

參考文獻(xiàn)：Isabella VM, Ha BN, Castillo MJ, Lubkowicz DJ, Rowe SE, Millet YA, Anderson CL, Li N, Fisher AB, West KA, Reeder PJ, Momin MM, Bergeron CG, Guilmain SE, Miller PF, Kurtz CB, Falb D. Development of a synthetic live bacterial therapeutic for the human metabolic disease phenylketonuria. Nat Biotechnol. 2018 Oct;36(9):857-864.